Month: September 2022

FUTILE SEARCH. "The failure of grafted normal cells to replicate indefinitely in vivo means that, given the "perfect" conditions of nutrition and environment that an isologous animal represents, transplanted normal cells are still incapable of immortality. This leads to the further conclusion that a search for perfect in vitro conditions in which normal cells might reveal immortality is futile." http://protein.bio.msu.ru/biokhimiya/contents/v62/full/62111380.html View in LinkedIn

"THE FINITUDE of normal cell replication has been substantially demonstrated to occur both in vivo and in vitro." http://protein.bio.msu.ru/biokhimiya/contents/v62/full/62111380.html View in LinkedIn

THERE ARE NO IMMORTAL NORMAL CELLS. "As a result of our demonstration that normal cells are mortal, the focus of attention in the field of aging began to be directed to the cell itself, and the dogma of fifty years duration began to die a slow death." http://protein.bio.msu.ru/biokhimiya/contents/v62/full/62111380.html View in LinkedIn

CANCER IMMORTALITY. "But, is it really true that cell immortality exists in vitro? Is HeLa, for example, immortal? In fact, it has never been proven." http://protein.bio.msu.ru/biokhimiya/contents/v62/full/62111380.html View in LinkedIn

UNPROVEN. "No one has ever demonstrated that an allegedly immortal cell line, like HeLa, is actually immortal, that is that individual HeLa cells undergo continuous cell divisions without periodic exchange or reorganization of genetic information." http://protein.bio.msu.ru/biokhimiya/contents/v62/full/62111380.html View in LinkedIn

PREREQUISITE OF IMMORTALITY. "The importance of this point is that immortalization or transformation of a cell population may be dependent upon a gene, virus, chemical, or radiation source that confers upon normal cells the ability to exchange or rearrange genetic information which appears to be the prerequisite for population immortality." http://protein.bio.msu.ru/biokhimiya/contents/v62/full/62111380.html View in LinkedIn

WORTH REPEATING. "The ability to exchange or rearrange genetic information...appears to be the prerequisite for population immortality." http://protein.bio.msu.ru/biokhimiya/contents/v62/full/62111380.html View in LinkedIn

CONCLUSION #1. "Inverse Relationship between Donor Age and Population Doublings. In 1965 we reported that cultured fibroblasts derived from older humans replicate less than those derived from embryos." http://protein.bio.msu.ru/biokhimiya/contents/v62/full/62111380.html View in LinkedIn

CONCLUSION #2. "Changes That Precede Phase III. More than two hundred changes in biological activity have been shown to occur in cultured normal human fibroblasts as they age in vitro. Of great importance is that many of these changes are identical to the changes recognized as characteristic of aging in intact humans." http://protein.bio.msu.ru/biokhimiya/contents/v62/full/62111380.html View in LinkedIn

CONCLUSION #3. "Direct Relationship between Species Lifespan and Population Doublings of Their Cultured Normal Cells. Several years ago we suggested that the population doubling potential of cultured fibroblasts from several animal species revealed a surprisingly good direct correlation with maximum species lifespan." http://protein.bio.msu.ru/biokhimiya/contents/v62/full/62111380.html View in LinkedIn