Year: 2022

OUTPUT. “Moreover, the signal response surfaces of the AND and NAND gates (Figs 3 and 5) show the sigmoid response property for both inputs, largely due to the high cooperativity of the two transcriptional activator proteins.” https://lnkd.in/dsEUY4ZP View in LinkedIn

STUNNING. “Millions of animals have only thirty-five basic body plans.” https://lnkd.in/d327viEM View in LinkedIn

“René Binet’s Esquisses Décoratives contains a sequence of architectural designs based on the biological and morphological illustrations found in Ernst Haeckel’s well-known ‘Art Forms in Nature’. “ https://lnkd.in/dHW69JpP View in LinkedIn

OPTIMAL SIGNAL. “The sigmoidal response filtering property can suppress the analogue noise in biomolecular logic systems and increase signal robustness, enhancing the error tolerance capability and the scalability of the logic gates.” https://lnkd.in/dsEUY4ZP View in LinkedIn

FLEXIBILITY. “Having a similar role as their electronic counterparts, the engineered AND and NAND gates are the fundamental components in designing biologically based digital systems to control cell signalling and can be easily applied in different contexts, owing to their modularity and orthogonality, to implement human-designed intra- and extra-cellular control functions.” https://lnkd.in/dsEUY4ZP View in LinkedIn

APPROACH. “Here the genetic logic devices were constructed using a forward engineering approach consisting of quantitative in-context characterization, computational modelling, construction and testing.” https://lnkd.in/dsEUY4ZP View in LinkedIn

MILIEU. “The results obtained show that both the abiotic and genetic contexts (for example, chassis, medium, temperature and embedded sequence context) in which the parts and modules are placed have a significant impact on their functionality.” https://lnkd.in/dsEUY4ZP View in LinkedIn

REPRODUCIBILITY. “However, the behaviours of assembled logic circuits were modelled predictably from components when characterized in the same abiotic and genetic context as required for their final operation, implying that, to achieve predictable circuit engineering, individual parts and modules should be in-context characterized to eliminate or reduce any behavioural variations arising from differences in context.” https://lnkd.in/dsEUY4ZP View in LinkedIn

STRATEGY. “The obtained transfer functions of the logic gates implemented here can be used to simulate the behaviour of larger integrated devices or systems when the context in which they are placed is preserved. Alternatively, biological components might be insulated physically and genetically to let them act more stably and predictably in different contexts.” https://lnkd.in/dsEUY4ZP View in LinkedIn

SCALABLE. “Orthogonal parts and modules, such as the AND gate engineered in this study, are important for the compatibility and scalable design of large gene circuits comprising many components. Orthogonality implies that the newly added parts and modules should not cross-talk with those present in the engineered biological systems as well as the host genetic background.” https://lnkd.in/dsEUY4ZP View in LinkedIn