N2 FIX BYPASS. “The Klebsiella pneumoniae nif gene cluster, divided into seven operons, is one of the most studied systems. Its nitrogen fixation capacity is subject to complex cascade regulation and physiological limitations. The entire K. pneumoniae nif gene cluster was reassembled…(in) BioBrick parts in Escherichia coli. It provided ~100% activity of native K. pneumoniae system.” The engineered system “bypassed the original complex regulatory circuits,” and replaced it with a simple system. Promising work. ”
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http://www.ncbi.nlm.nih.gov/m/pubmed/23935879/