Month: September 2022

GENOME-PHENOTYPE LINKS. "Even when identified, it is often unclear how a causal mutation perturbs the function of the corresponding gene or gene product. To “connect the dots” of the genomic revolution, functions and context must be assigned to large numbers of genotypic changes." http://www.sciencedirect.com/science/article/pii/S0092867414014226 View in LinkedIn

GIANT TASK. "Even considering only one splice variant per gene, approximately 20,000 protein-coding genes must be handled and ∼200 million protein pairs tested to generate a comprehensive binary reference PPI map. Even with nearly complete, high-quality reference interactome maps of biophysical interactions, how can the biological relevance of each interaction be evaluated under physiological conditions?" (PPI = Protein-Protein Interactions). http://www.sciencedirect.com/science/article/pii/S0092867414014226 View in LinkedIn

COMMUNITY OF LIVING MACHINES. "The properties of living cells are mediated by a huge number of ever-changing interactions of their component macromolecules forming living machines; collectively, these are termed the interactome. Pathogenic alterations in interactomes mechanistically underlie diseases." https://lnkd.in/eVepjNC View in LinkedIn

COMMUNITY OF ASSOCIATIONS. "There is no single, fixed human interactome. Instead, it is likely that the number of interaction networks might number in the thousands, perhaps even an infinite number. There is no single human interactome. There are many. " https://genomebiology.biomedcentral.com/articles/10.1186/s13059-016-0913-4 View in LinkedIn

VAST PROTEIN INTERACTIONS. "Proteins are the central players in interactomes. Most eukaryotic cells express several thousand proteins, and multicellular organisms commonly express tens of thousands of proteins, exponentially increasing the probable total number of macromolecular interactions in these organisms, all passing information on pathways linking each other throughout the cell. For example, analyses suggest on the order of 50,000 different protein interaction pairs, and perhaps four times more in humans." https://lnkd.in/eVepjNC View in LinkedIn

50,000 INTERACTIONS. "This number is much higher if one considers the many possible combinations of interactions any protein may make at different times, or if one includes interactions between proteins and other macromolecules (e.g., DNA and RNA). A major challenge in cell biology is thus to elucidate dynamic interactomes of consequence and to understand how these interactomes lead to cellular phenotypes." https://lnkd.in/eVepjNC View in LinkedIn

82 MILLION PROTEIN PAIRS. "To identify these interactions, the researchers used a high-throughput yeast two-hybrid approach, in which 82 million protein pairs were each tested four times in two different configurations for their ability to activate a reporter gene in yeast." https://lnkd.in/etGh98E View in LinkedIn

CONSERVED PATTERNS. "The networks of protein–protein interactions, or interactomes, although distinctive to their individual organisms, manifest global and local characteristics that are shared across species. Most notably, the organization of these networks exhibits a scale-free topology with a substantial number of highly connected hub proteins." https://lnkd.in/eTyvTV7 View in LinkedIn

VISUAL DESCRIPTION of the protein-protein interactome complexity. https://lnkd.in/eA-DhWV View in LinkedIn

COLLABORATIVE GROUPS. "As proteins form larger assemblies to confer context-specific functionality, the systematic analysis of protein–protein interactions (PPIs) is a powerful strategy for identifying physiological pathways associated with a protein of interest, following the “guilt of association” principle, and to further characterize these pathways by unveiling the physical contacts that underlie them." https://lnkd.in/eA-DhWV View in LinkedIn