Month: September 2022

DOUBLE MOTHER MAMMALS. "Given the successful establishment of parthenogenetic haploid embryonic stem cells in the mouse, rat and monkey, we expect that our approach can also be applied in other mammalian species to produce bimaternal animals." https://lnkd.in/gCT7Dcz View in LinkedIn

BIOLOGICAL IMPROVEMENTS. "Homozygous genotypes have great potential in breeding and genomic researches in higher plants. Induction of parthenogenesis via pollination with gamma-irradiated pollens is an efficient method to obtain haploid plants that can overcome the biological limitations of fruit trees." https://lnkd.in/g8XbsX2 View in LinkedIn

HYBRID HAPLOID TREES. "In this study, we investigated the induction of haploid plants in ‘Hirado Buntan’ pummlo (Citrus grandis) through in-situ gynogenesis by pollination with irradiated pollens of trifoliate orange (255, 325 and 500 Gy) and ‘Tongshui 72-1 Jincheng’ sweet orange (255, 395 and 500 Gy)." http://www.sciencedirect.com/science/article/pii/S0304423816302710 View in LinkedIn

GAMETE MANIPULATION. "Diploidy is a fundamental genetic feature in mammals, in which haploid cells normally arise only as post-meiotic germ cells that serve to ensure a diploid genome upon fertilization. Gamete manipulation has yielded haploid embryonic stem (ES) cells from several mammalian species but haploid human ES cells have yet to be reported." https://lnkd.in/gQeEBUz View in LinkedIn

HAPLOID HUMAN STEM CELLS. "Here we generated and analysed a collection of human parthenogenetic ES cell lines originating from haploid oocytes, leading to the successful isolation and maintenance of human ES cell lines with a normal haploid karyotype. Haploid human ES cells exhibited typical pluripotent stem cell characteristics, such as self-renewal capacity and a pluripotency-specific molecular signature." (ES = embryonic stem cells). https://lnkd.in/gQeEBUz View in LinkedIn

HUMAN HAPLOIDS. "Generation of human haploid embryonic stem cells from parthenogenetic embryos obtained by microsurgical removal of male pronucleus." https://lnkd.in/gxKpzTq View in LinkedIn

PARTHENOGENIC MAMMALIAN EMBRYOS. "Parthenogenetic haploid embryonic stem cells efficiently support mouse generation by oocyte injection. Mammalian haploid embryonic stem cells (haESCs) have been recently generated from parthenogenetic and androgenetic embryos. Both parthenogenetic haESCs (PG-haESCs) and androgenetic haESCs (AG-haESCs) can be used for cell-based reverse and forward genetic screens on a whole-genome scale." https://lnkd.in/gdkjfHz View in LinkedIn

“ZEBRA SHARK surprises scientists by giving birth without male.” http://www.cnn.com/2017/01/17/health/zebra-shark-reproduction/index.html View in LinkedIn

PARTHENOGENIC PLANT EMBRYOS. "Induced Parthenogenesis with an Apomict-Derived Gene. Haploid plants can be recovered from parthenogenetically-derived embryos. This gene has potential utility for haploid induction through parthenogenesis in reduced eggs or apomixis through induction of parthenogenesis in unreduced eggs." https://pag.confex.com/pag/xxiv/webprogram/Paper18705.html View in LinkedIn

"GENOME EDITING Using Mammalian Haploid Cells. Haploid cells are useful for studying gene functions because disruption of a single allele can cause loss-of-function phenotypes. Recent success in generating haploid embryonic stem cells (ESCs) in mice, rats, and monkeys provides a new platform for simple genetic manipulation of the mammalian genome. Use of haploid ESCs enhances the genome-editing potential of the CRISPR/Cas system." http://www.mdpi.com/1422-0067/16/10/23604/htm View in LinkedIn