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COMPLEX MODULARITY. “The device integrated two individual environmental signals through the two transcriptional inputs to generate one output response, accordingly, in the logic AND manner. The AND gate was shown to be modular by exchanging the inputs and output of the circuit while preserving the logic AND function.” https://lnkd.in/dsEUY4ZP View in LinkedIn

EXAMPLE. “A modular and orthogonal genetic AND gate was constructed in E. coli using a new strictly regulated orthogonal system from P. syringae. The hrpR/hrpS hetero-regulation of the σ54-dependent transcription allowed the AND gate to be tightly controlled with a robust near-digital logic behaviour.” https://lnkd.in/dsEUY4ZP View in LinkedIn

ENGINEERED CHASSIS. “Hence, a pressing need is to extend this limited parts list, for example, by exploiting and re-engineering the diverse, naturally evolved orthogonal biological control modules in many organisms.” https://lnkd.in/dsEUY4ZP View in LinkedIn

THE CHALLENGE. “Nevertheless, the effort in designing modular and orthogonal genetic devices is largely constrained by the fact that there are only a limited number of orthogonal regulatory components in the current toolbox of gene circuit engineering, such as transcription factor tetR, cI, luxR genes and their regulatory promoters.” https://lnkd.in/dsEUY4ZP View in LinkedIn

IDEAL STRUCTURE. “Ideally, a genetic logic device should be modular and orthogonal to their host chassis to facilitate its reuse and reliability in different contexts.” https://lnkd.in/dsEUY4ZP View in LinkedIn

STRATEGY. “The obtained transfer functions of the logic gates implemented here can be used to simulate the behaviour of larger integrated devices or systems when the context in which they are placed is preserved. Alternatively, biological components might be insulated physically and genetically to let them act more stably and predictably in different contexts.” https://lnkd.in/dsEUY4ZP View in LinkedIn

My tiny crab who lives on an anemone is filter feeding with his extended mouthpiece. View in LinkedIn

REPRODUCIBILITY. “However, the behaviours of assembled logic circuits were modelled predictably from components when characterized in the same abiotic and genetic context as required for their final operation, implying that, to achieve predictable circuit engineering, individual parts and modules should be in-context characterized to eliminate or reduce any behavioural variations arising from differences in context.” https://lnkd.in/dsEUY4ZP View in LinkedIn

BEYOND THE SKULL. “As Lewis Carroll's Alice might have phrased it: ‘You're nothing but a pack of neurons’.’ But it seems to me that this is not so astonishing a statement for a scientist to make. Isn't this what reductionist science has always believed?”" " https://lnkd.in/d5mx5w22 View in LinkedIn

MILIEU. “The results obtained show that both the abiotic and genetic contexts (for example, chassis, medium, temperature and embedded sequence context) in which the parts and modules are placed have a significant impact on their functionality.” https://lnkd.in/dsEUY4ZP View in LinkedIn