Category: Uncategorized

NEEDING EVIDENCE. "As stated by others, it is imperative that those who claim that the vast majority of intergenic transcription is functional test their hypotheses. In the absence of this evidence, the declaration that we are in the midst of a paradigm shift with regards to eukaryotic genomes and gene expression seems premature." http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1004351 View in LinkedIn

SEVENTY-FIVE PERCENT. "In 2012, as part of the ENCODE project, which focused initially on humans, Djebali et al., showed the staggering complexity and expanse of the human transcriptome. At least three-quarters of the genome produces transcripts, many of which demonstrate complex splicing and specific expression patterns." https://lnkd.in/e9BHyYj View in LinkedIn

MINOR TRANSCRIPTION. "Eukaryotes appear to have evolved an intricate network of coupling reactions that are required to cope with a large burden of junk RNA. These findings are consistent with the idea that eukaryotic genomes are filled with junk DNA that is transcribed at a low level." http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1004351 View in LinkedIn

INCOMPLETE CATALOG. "The application of deep sequencing to RNA analysis has shown that the majority of the genomic sequence is transcribed in humans and other metazoan species. These analyses have resulted in the identification of thousands of novel coding and non-coding genes. Yet, it is clear that the full catalog of transcription remains elusive." https://lnkd.in/e9BHyYj View in LinkedIn

PROTECTIVE MARKER. "One would predict that lncRNAs contain a differential set of identity elements that not only serve to prevent their decay but also retain them in the nucleus. This would especially be critical for lncRNAs that are spliced." (LncRNAs = long non-coding RNAs). http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1004351 View in LinkedIn

MOSTLY TRANSCRIBED. "One of the most widely discussed discoveries of the past decade of transcriptome analysis is that much of the metazoan genome is transcribed at some level." Even non-coding ("junk" viral DNA). http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1004351 View in LinkedIn

DESTRUCTION. "Transcripts that lack identity elements are targeted for degradation. In agreement with this model, intronless RNA molecules that have a random sequence are poorly exported from the nucleus and have a very short half-life." http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1004351 View in LinkedIn

IDENTITY ELEMENTS. "These mRNA identity features activate coupling reactions, which in turn promote the further processing, nuclear export, and translation of a particular transcript. Likewise, other classes of functional RNAs (e.g., tRNAs and snRNAs) have their own identity elements." http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1004351 View in LinkedIn

CODING VS NON-CODING. "On average, protein-coding genes have eight introns, while the majority of annotated ENCODE intergenic transcripts tend not to be spliced." http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1004351 View in LinkedIn

COUPLING FUNCTION. "The ultimate goal of these coupling reactions is to sort protein-coding RNAs (i.e. mRNA) from intergenic transcripts." http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1004351 View in LinkedIn